Table 2.
Primer Pairs Sequence Cloning
| Primer | Sequence |
|---|---|
| sACex5v2-ex12v2 first F: | 5′-CGATGTGCCGGATTATGCTATGTCTCTCTCTGAAGG-3′ |
| sACex5v2-ex12v2 first R: | 5′-CGTCATCCTTGTAATCTTCTGGACTCAGCCTTG-3′ |
| sACex5v2-ex12v2 second F: | 5′-CGCTCTAGAGCCACCATGTATCCATACGATGTGCCGGATTAT-3′ |
| sACex5v2-ex12v2 second R: | 5′-CGCGGATCCCTTATCGTCGTCATCCTTGTAATC-3′ |
| sACex2-ex5 first F: | 5′-GATGTGCCGGATTATGCTATGAACACTCCAAAAGAAG-3′ |
| sACex2-xe5 (NheI) R: | 5′-GGGGCTAGCAGTGCATCACCTGCAAATTTC-3′ |
| sACex2-ex5 second F: | 5′-CGCTCTAGAGCCACCATGTATCCATACGATGTGCCGGATTAT-3′ |
| sACfl first F: | 5′-CGATGTGCCGGATTATGCTATGAACACTCCAAAAGAAGA-3′ |
| sACfl second F: | 5′-CGCTCTAGAGCCACCATGTATCCATACGATGTGCCGGATTAT-3′ |
| sACfl R: | 5′-CGCGGATCCCTTATCGTCGTCATCCTTGTAATC-3′ |
| sACex2-ex7 F: | 5′-CGCTCTAGAGCCACCATGTATCCATACGATGTGCCGGATTAT-3′ |
| sACex2-ex7 first R: | 5′-CGTCATCCTTGTAATCGGAGACTCTGGGACAC-3′ |
| sACex2-ex7 second R: | 5′-CGCGGATCCCTTATCGTCGTCATCCTTGTAATC-3′ |
Definition of abbreviations: F, forward primer; R, reverse primer; sAC, soluble adenylyl cyclase; sACfl, full-length sAC.
Bold letters indicate hemagglutinin tag sequence; bold italic letters indicate flag tag sequence; underlined letters indicate restriction site sequences.