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. Author manuscript; available in PMC: 2015 Jan 13.
Published in final edited form as: J Otol. 2012 Dec;7(2):92–101. doi: 10.1016/S1672-2930(12)50023-1

Figure 7.

Figure 7

Photomicrographs showing vestibular organotypic cultures from the middle area of macula of utricle after 48 h treatment with carboplatin in an adult chinchilla culture system. Hair cells are labeled with Alexa Fluor 488-phalloidin. Carboplatin concentrations are shown in each panel. (A) A normal control showing normal arrangement of vestibular hair cells. Vestibular culture treated with carboplatin at a concentration of 10 µM shows no damage of hair cells (B). However, carboplatin treatment at 50 µM and 100 µM for 48 h results in abnormal arrangement of vestibular hair cells in the macula of utricle (C, D). Cilia in some vestibular hair cells missing after 48 h of carboplatin treatment at 500 µM. (E). Massive damage in the macula of utricle when the concentrations of carboplatin exceeds 1000 µM (F, G, H).