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. 2015 Jan 13;5:7750. doi: 10.1038/srep07750

Figure 4. Cross-regulation of Hnf1b and Egr4.

Figure 4

(a) Injection of 100 pg hnf1bGR mRNA expands egr4 and mafb expression anteriorly, while repressing hoxb1 and fgf3 expression. (a) Injection of 100 pg egr4GR mRNA expands krox20 and mafb expression and represses hnf1b, hoxb1 and fgf3 expression. The graphs indicate the percentage of embryos with normal (white), reduced/lost (red) or expanded/ectopic (blue) gene expression. The number of embryos analyzed is indicated on top of each bar. (c) Similar results were obtained by injection of 100 pg mouse Egr4 (mEgr4GR) mRNA. (d) Embryos injected with 100 pg of mouse Krox20 (mKrox20GR) or Xenopus mafb (mafbGR) mRNAs exhibit a dramatic reduction of hnf1b and egr4 expression. (e) Two-color in situ hybriidization for egr4/hnf1b and krox20/hnf1b. Overtime, the anterior limit of hnf1b retracts posteriorly and segregates from the r5 egr4 and krox20 expression domains. Dorsal views, anterior to top. In all panels scale bars, 300 μm.