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VopQS200P does not induce vacuole fragmentation. (A) Serial dilutions of yeast strain BY4742 harboring pRS413-Gal1, pRS413-Gal1-VopQ, or pRS413-Gal1-VopQS200P in Complete Supplement Mixture (CSM) medium lacking histidine, supplemented with either 2% glucose or 2% galactose. (B) Vacuolar morphology of the BY4742 strain harboring pRS413-Gal1, pRS413-Gal1-VopQ, or pRS413-Gal1-VopQS200P was visualized via FM4-64 staining. (Scale bar, 5 μm.)
