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. 2014 Dec 22;112(1):232–237. doi: 10.1073/pnas.1422165112

Fig. 2.

Fig. 2.

Candidate target gene evaluation in OV8. (A) qRT-PCR analysis of OV8 cells after infection (MOI > 1) with each of the second best shRNAs and 48-h selection with Puromycin. For each gene, expression values are normalized by reference to that of the housekeeping gene, Tubulin, and are reported as a percentage of the levels obtained when infecting the cells with the control lentivirus, shLuc. An additional control lentivirus shCTRL is also reported. (B) Cell proliferation assay. OV8 cells were infected at MOI > 1 and selected for 48 h with Puromycin. OV8 cells used in this assay express luciferase. Cell number was titered by Luciferase expression immediately after selection (day 0) and 4 d later (day 4). For each shRNA is reported the fold increase in Luciferase signal over 4 d of culture. Where reported, error bars represent standard deviations of triplicate measurements. (C) GFP competition assay outline. (D) GFP competition assay results.