Table 1.
Expression of Oprm1 splice variants in different brain regions of CD-1 mice
| Normalized expression levels [E−(ΔCT) × 105] | ||||||||||
| Variant | Prefrontal cortex | Striatum | Thalamus | Hypothalamus | Hippocampus | PAG | Brainstem | Cerebellum | Spinal cord | Whole brain |
| 7TM variants (exons 1/2/3/variable) | ||||||||||
| mE1-2 | 532 ± 139 | 4,328 ± 1,010 | 2,721 ± 278 | 1,237 ± 412 | 4,429 ± 986 | 4,010 ± 848 | 6,019 ± 1,139 | 159 ± 38.4 | 2,612 ± 480 | 604 ± 108 |
| mMOR-1A | 96.2 ± 22 | 295 ± 67 | 614.1 ± 85 | 376 ± 93 | 657 ± 204 | 470 ± 67 | 579 ± 134 | 34.5 ± 8.6 | 186 ± 27 | 52.5 ± 9.3 |
| mMOR-1B1 | 139 ± 1.5 | 269 ± 8.3 | 1,104 ± 247 | 306 ± 24 | 80.1 ± 2.0 | 309 ± 59 | 308 ± 25 | 15.2 ± 0.2 | 205 ± 27 | 71.8 ± 1.8 |
| mMOR-1B2 | 8.4 ± 1.6 | 11.8 ± 0.6 | 23.5 ± 7.4 | 11.7 ± 3.4 | 5.6 ± 1.8 | 12.4 ± 1.0 | 15.0 ± 1.6 | 2.1 ± 0.7 | 6.6 ± 2.6 | 2.8 ± 1.0 |
| mMOR-1B3 | 29.6 ± 8.6 | 36.0 ± 1.8 | 68.9 ± 20 | 43.4 ± 11 | 139 ± 57 | 62.2 ± 11 | 95.5 ± 32 | 4.4 ± 1.7 | 59.7 ± 15 | 9.2 ± 2.4 |
| mMOR-1B4 | 62.1 ± 2.7 | 107 ± 23 | 226.5 ± 68 | 84.5 ± 29 | 624 ± 242 | 143 ± 42 | 169 ± 47 | 16.3 ± 7.6 | 197 ± 23 | 49.4 ± 9.0 |
| mMOR-1B5 | 28.7 ± 1.6 | 91.9 ± 17 | 117.9 ± 34 | 65.8 ± 19 | 61.1 ± 18 | 99.2 ± 9.5 | 110 ± 29 | 3.8 ± 1.8 | 49.9 ± 11 | 15.8 ± 4.2 |
| mMOR-1C | 41.9 ± 9.7 | 72.8 ± 17 | 156.3 ± 56 | 89.3 ± 43 | 142 ± 37 | 134 ± 15.2 | 171 ± 48 | 8.4 ± 2.6 | 140 ± 24 | 34.1 ± 0.8 |
| mMOR-1D | 0.7 ± 0.2 | 1.3 ± 0.5 | 2.7 ± 0.8 | 1.2 ± 0.03 | 7.2 ± 3.7 | 3.7 ± 0.9 | 9.4 ± 1.5 | 0.2 ± 0.03 | 1.4 ± 0.6 | 0.6 ± 0.3 |
| mMOR-1E | 31.4 ± 4.8 | 23.5 ± 4.8 | 17.5 ± 2.9 | 9.7 ± 1.8 | 72.0 ± 11 | 12.7 ± 4.7 | 14.5 ± 5.2 | 7.8 ± 0.6 | 6.9 ± 2.1 | 4.0 ± 1.3 |
| mMOR-1F | 11.9 ± 2.4 | 66.2 ± 3.7 | 101.7 ± 8.2 | 66.2 ± 12 | 8.9 ± 2.9 | 85.0 ± 14.9 | 103 ± 13 | 1.4 ± 0.3 | 13.9 ± 2.9 | 12.9 ± 3.7 |
| mMOR-1H* | 1.8 ± 0.6 | 2.2 ± 0.5 | 4.9 ± 1.2 | 2.0 ± 0.5 | 1.7 ± 1.0 | 2.1 ± 0.7 | 3.0 ± 1.1 | 0.1 ± 0.0 | 24.5 ± 8.9 | 1.1 ± 0.3 |
| mMOR-1i* | 14.7 ± 4.5 | 16.1 ± 0.8 | 47.1 ± 15 | 21.0 ± 4.9 | 11.8 ± 2.9 | 21.6 ± 1.7 | 21.1 ± 1.9 | 1.0 ± 0.2 | 20.0 ± 1.0 | 5.4 ± 1.1 |
| mMOR-1J* | 1.7 ± 0.6 | 2.5 ± 0.4 | 8.3 ± 2.8 | 3.5 ± 1.0 | 3.4 ± 1.0 | 2.6 ± 0.4 | 3.1 ± 0.8 | 0.1 ± 0.0 | 1.6 ± 0.3 | 0.7 ± 0.2 |
| mMOR-1O | 217 ± 64 | 101 ± 16 | 192 ± 77 | 29.4 ± 3.5 | 1,359 ± 201 | 64.1 ± 9.0 | 61.6 ± 12 | 23.4 ± 7.9 | 68.3 ± 14 | 84.6 ± 30 |
| mMOR-1P | 3.6 ± 0.4 | 7.9 ± 1.6 | 12.0 ± 2.1 | 5.4 ± 0.2 | 1.0 ± 0.7 | 5.3 ± 0.7 | 7.4 ± 0.5 | 0.2 ± 0.1 | 4.4 ± 1.0 | 1.6 ± 0.3 |
| mMOR-1U | 4.0 ± 1.3 | 5.9 ± 0.8 | 17.7 ± 5.5 | 8.0 ± 2.1 | 11.7 ± 3.5 | 6.1 ± 0.8 | 7.2 ± 1.8 | 0.3 ± 0.0 | 3.9 ± 0.8 | 1.9 ± 0.5 |
| mMOR-1W | 5.5 ± 1.7 | 8.0 ± 1.1 | 23.0 ± 6.9 | 10.6 ± 2.7 | 11.7 ± 3.1 | 8.2 ± 1.0 | 9.6 ± 2.4 | 0.4 ± 0.1 | 5.3 ± 1.1 | 2.6 ± 0.7 |
| 6TM variants (exons 11/2/3/variable) | ||||||||||
| mMOR-1G* | 21.1 ± 9.0 | 9.3 ± 1.2 | 11.0 ± 4.6 | 5.3 ± 1.7 | 4.6 ± 1.5 | 9.6 ± 2.3 | 15.6 ± 3.8 | 3.9 ± 1.3 | 65.2 ± 10.0 | 5.4 ± 1.5 |
| mMOR-1M* | 5.6 ± 1.8 | 3.5 ± 0.1 | 7.7 ± 1.2 | 3.5 ± 0.3 | 7.5 ± 1.6 | 2.6 ± 0.4 | 3.5 ± 1.2 | 0.9 ± 0.5 | 25.0 ± 6.1 | 1.0 ± 0.1 |
| mMOR-1N* | 0.3 ± 0.1 | 0.4 ± 0.1 | 0.5 ± 0.1 | 0.2 ± 0.03 | 0.9 ± 0.2 | 0.4 ± 0.1 | 0.5 ± 0.1 | 0.1 ± 0.02 | 1.5 ± 0.3 | 0.1 ± 0.03 |
| mMOR-1K* | 17.4 ± 4.5 | 11.2 ± 5.5 | 38.3 ± 1.4 | 9.0 ± 1.6 | 96.1 ± 24 | 27.3 ± 3.9 | 36.2 ± 3.4 | 1.1 ± 0.3 | 16.4 ± 5.0 | 3.6 ± 0.6 |
| mMOR-1L* | 8.0 ± 1.9 | 8.2 ± 0.6 | 23.3 ± 4.9 | 10.1 ± 0.7 | 10.0 ± 3.4 | 22.9 ± 2.9 | 21.1 ± 3.7 | 1.0 ± 0.1 | 10.7 ± 3.4 | 2.2 ± 0.6 |
| 1TM variants (exons 1/variable) | ||||||||||
| mMOR-1Q | 14.2 ± 2.5 | 51.6 ± 9.1 | 99.4 ± 18 | 44.8 ± 9.7 | 29.8 ± 9.4 | 71.3 ± 10.5 | 72.0 ± 13 | 1.4 ± 0.6 | 32.1 ± 5.0 | 8.3 ± 1.7 |
| mMOR-1R | 6.4 ± 1.9 | 9.3 ± 1.2 | 26.2 ± 7.7 | 12.3 ± 3.0 | 24.8 ± 5.9 | 9.6 ± 1.2 | 11.2 ± 2.7 | 0.5 ± 0.1 | 6.2 ± 1.2 | 3.1 ± 0.8 |
| mMOR-1S | 12.0 ± 3.0 | 11.9 ± 0.4 | 30.2 ± 2.7 | 15.7 ± 3.7 | 104.2 ± 12.8 | 39.0 ± 11.7 | 16.2 ± 6.5 | 1.6 ± 0.2 | 12.3 ± 2.3 | 5.2 ± 0.4 |
| mMOR-1T* | 7.5 ± 1.7 | 18.0 ± 0.6 | 31.1 ± 8.8 | 17.6 ± 2.8 | 23.5 ± 4.0 | 33.6 ± 8.4 | 25.0 ± 5.7 | 1.0 ± 0.4 | 3.8 ± 1.4 | 3.0 ± 0.5 |
| mMOR-1Z | 2.6 ± 0.8 | 3.8 ± 0.6 | 12.1 ± 3.9 | 5.2 ± 1.4 | 3.6 ± 1.4 | 4.0 ± 0.5 | 4.7 ± 1.2 | 0.1 ± 0.03 | 2.5 ± 0.6 | 1.3 ± 0.2 |
*
Generated through the exon 11 promoter.