Fig. 1.

Bag6 has distinct binding sites for TRC35 and Ubl4A at its C terminus. (A) Scheme of the five different Bag6 fragments and of the subfragments of Bag6E, which is divided further into the N terminus (E_N), the NLS (E_NLS), and a fragment containing only the putative BAG domain (E_BAG). (B, Left) Yeast two-hybrid assay between Bag6 fragments and either TRC35 or Ubl4A. The A fragment contains the UBL domain, and the E fragment contains the NLS and putative BAG domain. (Right) Yeast two-hybrid assay of TRC35 or Ubl4A and the Bag6 E fragments.