Fig. 2.

Defining the interaction between Bag6 and TRC35 by yeast two-hybrid assay. (A, Upper) Diagram of the Get4/Get5-N complex (PDB ID code 3LKU). Get4 is shown in gray and red, and Get5 is shown in green. (Lower) A top-view schematic representation of the architecture. The two β-strands in Get4 that are missing in TRC35 are outlined in blue. Ubl4A does not contain a Get5-N equivalent. (B) Bag6E fragments containing the activating domain were combined with full-length TRC35 (FL), TRC35-N (residues 1–157), or TRC35-C (residues 158–327) conjugated to the binding domain. TRC35-N and TRC35-C were defined by sequence alignment with Get4.