Fig. 3.

Transient ETV2 expression is sufficient to directly convert part of HAFs into ETVECs. (A) Dox-inducible ETV2 and rtTA-transduced HAFs were cultured in the presence of 100 ng/mL Dox for 21 d. The sorted CD31⁺ cells were cultured for another 10 d in the presence or absence of Dox. CD31^hi cells were sorted again, then cultured for an additional 10 d under the same culture conditions. Numbers on the contour plots indicate the percentage of cells under a Dox-free culture condition. (B) At 20 d after the Dox withdrawal, CD31^hi and CD31⁻ cells were subjected to quantitative RT-PCR. Gene expression levels relative to HPRT1 (mean ± SD; triplicate). (C) Photos of the two representative EC colonies at 20 d after the culture with or without Dox. Data are representative of three independent cell cultures. Hi, CD31^hi cells; Neg, CD31⁻ cells. (Scale bar: 50 μm.)