Skip to main content
. 2014 Dec 18;112(1):E21–E29. doi: 10.1073/pnas.1417015112

Fig. 2.

Fig. 2.

The combined inhibition of GLS and Hsp90 results in decreased viability and morphological changes of Tsc2−/− MEFs. (A) Tsc2−/− MEFs were treated with DMSO, 17AAG (0.5 μM), rapamycin (20 ng/mL), and BPTES (10 μM) as indicated for 48 and 72 h. Phase microscopy was used to observe cell viability. (B) Transmission electron microscopy (TEM) images (9,300 × 1.4×) of Tsc2−/− MEFs after 24 h of treatment with DMSO, 17AAG (0.5 μM), BPTES (10 μM), or BPTES plus 17AAG. Black arrows indicate mitochondria, and asterisks indicate lipid droplets. (C) TEM image (23,000 × 1.4×) showing a double-membrane autophagosome in Tsc2−/− MEFs treated with combined BPTES (10 μM) and 17AAG (0.5 μM). (D) TEM images depicting a lysosome (L) and late endosome (LE) in Tsc2−/− MEFs treated with combined BPTES (10 μM) and 17AAG (0.5 μM).