Skip to main content
. 2014 Dec 18;112(1):E21–E29. doi: 10.1073/pnas.1417015112

Fig. 4.

Fig. 4.

GDH inhibition does not sensitize cells to Hsp90 inhibition. (A) Cell death of Tsc2−/− MEFs treated with the indicated compounds for 72 h. The mean is shown; error bars represent SEM (n > 3). (B) Immunoblot analysis of cleaved PARP and α-tubulin in Tsc2−/− MEFs treated as in A for 24 h. (C) Intracellular glutamate levels in Tsc2−/− MEFs treated with BPTES (10 μM) for 72 h. A siRNA targeting GLS was used as a control. The mean is shown; error bars represent SEM (n = 3). (D) Tsc2−/− MEFs were treated with DMSO, 17AAG (0.5 μM), BPTES (10 μM), and glutamate (4 mM) as indicated for 48 h. Phase microscopy was used to observe cell viability. (E and F) Immunoblot analysis of cleaved PARP and α-tubulin in Tsc2−/− MEFs treated with the indicated compounds for 24 h. The concentrations used for the compounds were BPTES (10 μM), glutamate (4 mM), pyruvate (1 mM), and DM-αKG (7 mM).