Table 1. Proteins differently expressed between lesions from CL patients and normal skin.
|
Theoretical value |
Experimental value1 |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Spot no. | Gene Ontology | Protein name | NCBI accession no. | MW (kDa) | pI | MW(kDa) | pI | Probability | Z-value | Matched peptide | Coverage (%) | Expression change | |
| 1 | TTC37 | Tetratricopeptide repeat domain 37 | 7662078 | 177.5 | 7.7 | 210 | 9.5 | 1.0e+000 | 2.24 | 14 | 13 | Upregulated | |
| 2 | FBF1 | Fas (TNFRSF6) binding factor 1 | 23270771 | 90.58 | 6.7 | 100 | 9.5 | 9.0e-001 | 1.05 | 8 | 11 | Upregulated | |
| 3 | CDK11A | Cyclin-dependent kinase 11A | 66267414 | 53.91 | 4.8 | 51 | 8.2 | 1.0e+000 | 2.24 | 4 | 13 | Unique CL | |
| 4 | AIDA | Chromosome 1 open reading frame 80 | 119626771 | 35.08 | 6.2 | 55 | 7.3 | 1.0e+000 | 2.43 | 6 | 18 | Upregulated | |
| 5 | FAM13C | FAM13C | 119574574 | 55.44 | 6.5 | 50 | 6.8 | 1.0e+000 | 2.43 | 11 | 16 | Unique CL | |
| 6 | CASP9 | Caspase-9 | 158257592 | 47.09 | 5.7 | 40 | 8.3 | 1.0e+000 | 2.43 | 4 | 11 | Upregulated | |
| 7 | RABL2A | RAB, member of RAS oncogene family-like 2A | 220675523 | 18.52 | 4.4 | 25 | 9.2 | 1.0e+000 | 2.34 | 4 | 27 | Unique CL | |
| 8 | TMEM40 | Transmembrane protein 40 | 31542667 | 25.59 | 5.3 | 37 | 8.2 | 1.0e+000 | 2.43 | 4 | 23 | Unique CL | |
| 9 | GAPVD1 | GAPVD1 | 21739944 | 38.70 | 6.3 | 30 | 7.8 | 9.0e-001 | 1.57 | 6 | 15 | Unique CL | |
| 10 | IGBP1 | Immunoglobulin (CD79A) binding protein 1 | 193786113 | 39.28 | 5.2 | 36 | 7.3 | 1.0e+000 | 2.43 | 7 | 22 | Unique CL | |
| 11 | MIF4GD | MIF4G domain-containing protein isoform 1 | 335334986 | 30.70 | 5.2 | 30 | 7.2 | 1.0e+000 | 2.42 | 5 | 21 | Upregulated | |
| 12 | BBOX1 | Gamma-Butyrobetain, 2-Oxoglutarate Dioxygenase 1 | 295982624 | 45.30 | 6.3 | 35 | 5.5 | 1.0e+000 | 2.43 | 6 | 19 | Unique CL | |
| 13 | AKIR1N1 | AKIR1N1 | 119627688 | 18.01 | 7.2 | 15 | 5.8 | 1.0e+000 | 2.25 | 4 | 35 | Unique CL | |
| 14 | CHKA | Choline kinase-α | 118137437 | 45.59 | 6.5 | 41 | 5.4 | 1.0e+000 | 2.41 | 7 | 19 | Unique CL | |
| 15 | METTL10 | METTL10 | 57997124 | 32.13 | 5.8 | 5.0 | 4.3 | 1.0e+000 | 2.05 | 7 | 14 | Unique CL | |
| 16 | TRB | TCR-β | 54292535 | 5.58 | 6.8 | 17 | 4.5 | 1.0e+000 | 2.43 | 4 | 46 | Upregulated | |
| 17 | HAGH | HAGH | 158261333 | 29.19 | 6.5 | 33 | 4.7 | 1.0e+000 | 2.43 | 5 | 27 | Unique CL | |
| 18 | IL12RB1 | Interleukin-12 receptor subunit beta-1 | 24497440 | 43.38 | 6.8 | 40 | 4.5 | 1.0e+000 | 2.43 | 9 | 28 | Unique CL | |
| 19 | SNRNP48 | SNRNP48 | 11957561 | 41.82 | 6.0 | 33 | 4.0 | 1.0e+000 | 1.30 | 6 | 11 | Unique CL | |
| 20 | BRF1 | Transcription factor IIIB 90 kDa | 338753412 | 48.36 | 4.8 | 36 | 3.9 | 1.0e+000 | 2.43 | 7 | 17 | Upregulated | |
| 21 | HAUS5 | HAUS augmin-like complex subunit 5 | 39963101 | 32.91 | 7.2 | 38 | 3.7 | 1.0e+000 | 1.70 | 6 | 25 | Unique CL | |
| 22 | KIR2DL4 | Killer cell receptor | 39963101 | 36.05 | 6.2 | 55 | 5.5 | 1.0e+000 | 2.17 | 3 | 15 | Unique CL | |
| 23 | FKBP4 | FKBP4 | 4503729 | 52.07 | 5.3 | 40 | 3.5 | 1.0e+000 | 2.43 | 8 | 16 | Unique CL | |
| 24 | ATL1 | Atlastin GTPase 1 | 4406632 | 54.50 | 5.8 | 50 | 5.5 | 1.0e+000 | 2.43 | 4 | 13 | Unique CL | |
| 25 | BCAM | Basal cell adhesion molecule | 342196430 | 68.17 | 5.5 | 55 | 3.8 | 1.0e+000 | 2.43 | 7 | 13 | Downregulated | |
| 26 | UBR1 | Ubiquitin protein ligase E3 component n-recognin 1 | 14042752 | 95.40 | 5.8 | 55 | 5.0 | 1.0e+000 | 0.89 | 11 | 15 | Unique CL | |
| 27 | KPNA1 | Karyopherin-α 1 | 222144293 | 60.95 | 4.9 | 53 | 3.6 | 1.0e+000 | 2.43 | 6 | 13 | Unique CL | |
| 28 | DARS | Aspartyl-tRNA synthetase | 45439306 | 57.52 | 6.1 | 52 | 5.5 | 1.0e+000 | 2.43 | 8 | 17 | Unique CL | |
| 29 | cTAGE4 | cTAGE family, member 4 | 193290160 | 88.31 | 5.2 | 66 | 5.6 | 1.0e+000 | 0.87 | 13 | 19 | Upregulated | |
| 30 | TBC1D9B | TBC1D9B | 193786679 | 64.56 | 4.7 | 70 | 3.5 | 1.0e+000 | 1.16 | 7 | 15 | Unique CL | |
| 31 | ATP1A2 | Na(+)/K(+)-ATPase | 193785194 | 73.51 | 5.6 | 65 | 5.8 | 1.0e+000 | 2.43 | 7 | 16 | Upregulated | |
| 32 | RHPN2 | Hypothetical protein Rhophilin-2 | 21732479 | 74.40 | 6.5 | 72 | 5.6 | 1.0e+000 | 2.43 | 5 | 9 | Unique CL | |
| 33 | SKIL | SKI-like isoform 3 | 223029422 | 76.02 | 6.6 | 75 | 5.6 | 1.0e+000 | 2.04 | 7 | 7 | Unique CL | |
| 34 | RNF40 | Ring finger protein 40 | 7662230 | 114.4 | 6.0 | 90 | 4.2 | 1.0e+000 | 2.43 | 10 | 10 | Unique CL | |
| 35 | ANKLE2 | Ankyrin repeat and LEM domain-containing protein 2 | 148664230 | 104.95 | 6.7 | 95 | 3.8 | 1.0e+000 | 1.53 | 7 | 13 | Downregulated | |
| 36 | MYPBC1 | Hypothetical protein MYBPC1 | 30722349 | 88.87 | 5.9 | 150 | 3.5 | 1.0e+000 | 0.91 | 9 | 12 | Unique CL | |
| 37 | PDE4DIP | Phosphodiesterase 4D | 30268245 | 114.62 | 5.0 | 90 | 4.5 | 1.0e+000 | 1.27 | 7 | 8 | Downregulated | |
| 38 | SLC8A1 | SLC8A1 | 163914373 | 105.58 | 4.9 | 95 | 4.7 | 1.0e+000 | 1.88 | 5 | 5 | Downregulated | |
| 39 | SART1 | Squamous cell carcinoma antigen recognized by T cells | 62897593 | 90.42 | 6.0 | 96 | 5.0 | 1.0e+000 | 2.43 | 11 | 15 | Unique CL | |
| 40 | PTPN5 | PTPN5 | 221039972 | 59.64 | 5.0 | 75 | 5.5 | 1.0e+000 | 2.43 | 7 | 8 | Unique CL | |
| 41 | NEK11 | Serine/threonine-protein kinase Nek11 | 41281753 | 54.56 | 5.7 | 51 | 6.3 | 1.0e+000 | 2.43 | 6 | 16 | Unique CL | |
| 42 | ZBTB10 | ZBTB10 | 119607481 | 74.92 | 4.8 | 69 | 8.0 | 1.0e+000 | 20.7 | 5 | 8 | Unique CL | |
| 43 | SNX25 | SNX25 | 193787897 | 83.27 | 5.9 | 70 | 6.8 | 1.0e+000 | 2.43 | 8 | 14 | Unique N. skin | |
| 44 | SPECC1 | SPECC1 | 21706968 | 55.18 | 5.1 | 64 | 8.2 | 1.0e+000 | 2.43 | 12 | 18 | Unique N. Skin | |
| 45 | CENPE | Centromere-associated protein E | 67464447 | 39.55 | 7.0 | 40 | 4.5 | 1.0e+000 | 1.21 | 8 | 29 | Unique N. Skin | |
| 46 | UBOX5 | RING finger protein 37 isoform B | 40806196 | 54.40 | 6.8 | 45 | 4.0 | 1.0e+000 | 2.27 | 5 | 11 | Unique N. Skin | |
| 47 | GNL1 | Guanine nucleotide binding protein-like 1 | 39645120 | 54.32 | 5.0 | 55 | 4.5 | 1.0e+000 | 2.43 | 6 | 10 | Unique N. Skin | |
| 48 | VPS51 | VPS51 | 38014611 | 79.57 | 6.6 | 58 | 4.5 | 1.0e+000 | 2.43 | 10 | 10 | Unique N. Skin | |
| 49 | ALDH2 | Mitochondrial aldehyde dehydrogenase 2 | 48256839 | 56.85 | 6.4 | 63 | 5.3 | 1.0e+000 | 2.43 | 6 | 15 | Unique N. Skin | |
| 50 | SYNE1 | Nesprin-1 | 73909082 | 59.51 | 6.5 | 70 | 5.4 | 1.0e+000 | 2.43 | 8 | 15 | Unique N. Skin | |
| 51 | DCHS2 | Protocadherin protein | 45439306 | 57.52 | 6.1 | 60 | 3.8 | 1.0e+000 | 2.43 | 8 | 13 | Unique N. Skin | |
| 52 | PALD1 | PALADIN | 20521820 | 98.25 | 6.1 | 98 | 5.5 | 1.0e+000 | 2.43 | 9 | 17 | Unique N. Skin | |
| 53 | HNRNPUL2 | Heterogeneous nuclear ribonucleoprotein U-like 2 | 118601081 | 85.66 | 4.9 | 90 | 5.3 | 1.0e+000 | 1.85 | 7 | 18 | Unique N. Skin | |
| 54 | ARHGEF7 | Rho guanine nucleotide exchange factor (GEF) 7 | 55957399 | 71.12 | 6.6 | 72 | 6.3 | 1.0e+000 | 2.43 | 6 | 7 | Unique N. Skin | |
| 55 | CCDC114 | Hypothetical protein FLJ32926 | 119572712 | 80.73 | 5.9 | 85 | 6.8 | 1.0e+000 | 1.53 | 9 | 17 | Unique N. Skin | |
| 56 | IPO7 | Importin-7 | 5453998 | 120.81 | 4.7 | 130 | 7.0 | 1.0e+000 | 1.13 | 10 | 18 | Unique N. Skin | |
| 57 | STK31 | Serine/threonine kinase 31 | 51095006a | 114.25 | 5.0 | 120 | 7.2 | 1.0e+000 | 2.43 | 9 | 13 | Unique N. Skin | |
| 58 | TNS3 | Tensin 3 variant | 62087570 | 137.54 | 6.4 | 121 | 7.4 | 1.0e+000 | 0.92 | 7 | 10 | Unique N. Skin | |
| 59 | ATP2B2 | ATPase, Ca2+ transporting, plasma membrane 3, isoform CRA_b | 119593265 | 128.19 | 5.7 | 119 | 7.6 | 1.0e+000 | 2.31 | 9 | 9 | Unique N. skin | |
Abbreviations: CL, cutaneous leishmaniasis; pI, isoelectric point, MW, molecular weight; N., normal; NCBI, National Center for Biotechnology Information.
Gene Ontology, protein name, NCBI accession number, theoretical value, probability, Z-value, matched peptide, and sequence coverage were obtained by search with Profound.
1
Experimental values were calculated from migration in 2D gels. The differences observed between the experimental and theoretical MW values (4, 15, 16, 26, and 36) were possibly due to the presence of isoforms or posttranslational modification of proteins.