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. 2014 Dec 8;1:48. doi: 10.3389/fmed.2014.00048

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Copyright © 2014 Tahan and Tahan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Human placenta layers: amnion, chorion, and decidua. Amniotic layer is composed of a single-celled epithelial layer and a deeper mesodermal layer. Chorionic layer is composed of a mesodermal layer and a trophoblast layer. (B) Isolation of amnion membrane from placenta. The maternal side of placenta is placed face down and a shallow X-shaped incision is made through the center of the placenta. The thin, nearly transparent amnion membrane is then peeled starting at the center of the cut and progressing outward. (C) Morphology of amniotic epithelial cells in culture (×40).