Figure 2.

HRAS-mutated spitzoid tumor in a female patient aged 47 years. The lesion was located on the right lower arm and measured 0.8 cm in diameter. The lesion was diagnosed elsewhere as STUMP (Spitzoid tumor of uncertain malignant potential). (A,B) The lesion was symmetrical, wedge shaped, mainly dermal located, with epithelioid spitzoid-looking cells in an eosinophilic hyalinized stroma [(A) HE 25×, (B) HE 400×]. There were 2 mitoses present per 1 mm². Deep mitoses were absent. (C) Sequence plot of the mutation HRAS c.182A >T(p.(Gln61Leu)) with 44% of mutant alleles. The genomic sequence of the gene investigated is marked in green bars, and the protein sequence information is on top of the gene sequence. The combined (forward and reverse) gene sequence information is highlighted in light green, and the expected protein sequence is placed on top. The forward sequence information is indicated as light blue bars and reverse sequence information as purple bars. The square box shows the number and percentage of the different nucleotides at the variant position; the red vertical bar indicates the HRAS hotspot mutation position. The sequence plots are generated in SeqNext (from JSI Medical Systems GmbH). Note that the HRAS RefSeq is NM_005343.2.