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. 2014 Oct 31;1:39. doi: 10.3389/fmed.2014.00039

Figure 4.

Figure 4

BRAF-mutated MBAIT lesion located on the posterior side of the ear in a 55-year-old female (Courtesy Dr. R. Kornegoor, Gelre Hospitals, Apeldoorn, The Netherlands). In (A), an overview showing a nodular dermal-based lesion with lymphocytes at the lateral margin. In this case, no common nevus component was present (HE, 25×). (B) A higher magnification demonstrates the large epithelioid aspect of the cells (HE, 200×). In (C), absence of BAP1 protein expression is seen in the large epithelioid melanocytes. Nuclei in the keratinocytes in the epidermis stain positive, serving as a positive internal control (400×). In (D), BRAF protein is expressed by the large epithelioid melanocytes (400×). (E) Sequence plots of the mutation BRAF c.1799T >A (p.(Val600Glu) alias p.V600E) with 23% mutant alleles and of the BAP1 (RefSeq NM_004656.2) c.581-1G >A splice site mutation with 34% mutant alleles. The sequence plots are visualized as described under Figure 2. In the BAP1 plot, the intron–exon boundary is represented as gray-white parts.