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. 2014 Aug 8;2(4):e964547. doi: 10.4161/21688362.2014.964547

Figure 1.

Figure 1.

Temporal Expression Analysis of Claudins during Mouse Kidney Development. (A) RT-PCR analysis of all claudin family members in E13.5 mouse kidneys. β-actin (β) was used as a positive control and RT-PCR reactions without the addition of RNA (lane 1) or with inactivated reverse transcriptase (‘—’; lanes 1 and 2) served as negative controls. The claudin family member is listed above each lane. Cldn18 was not amplified (data not shown). All RT-PCR amplicons were sequenced to confirm identity. (B) RT-PCR analysis of Cldn7, Cldn16, and Cldn19 at different stages of kidney development. β-actin was amplified as an internal control. Cldn7 and Cldn19 mRNA transcripts are expressed from embryonic day (E) 11.5 onwards, whereas Cldn16 mRNA transcripts are expressed from E12.5 onwards.