Table 1.
Claudin Primer Sequences used in RT-PCR
| Gene | Size | Name of primer | Sequence (5′ to 3′) |
|---|---|---|---|
| Claudin1 | 636 bp | mCldn1-`F | CCATGGCCAACGCGGGGCTGC |
| mCldn1-R | TCACACATAGTCTTTCCCAC | ||
| Claudin2 | 693 bp | mCldn2-F | CCATGGCCTCCCTTGGCGTCC |
| mCldn2-R | CACACATACCCAGTCAGGCTG | ||
| Claudin3 | 657 bp | mCldn3-F | CATATGTCCATGGGCTGG |
| mCldn3-R | TCAGACGTAGTCCTTGCGGT | ||
| Claudin4 | 633 bp | mCldn4-F | CCATGGCGTCTATGGGACTAC |
| mCldn4-R | TTACACATAGTTGCTGGCGG | ||
| Claudin5 | 420 bp | mCldn5-F | CCATGGGGTCTGCAGCGTTGG |
| mCldn5-R | GGCGAACCAGCAGAGCGGCAC | ||
| Claudin6 | 660 bp | mCldn6-F | CCATGGCCTCTACTGGTCTGC |
| mCldn6-R | TCACACATAATTCTTGGTGG | ||
| Claudin7 | 689 bp | mCldn7-F | CCATGGCCAACTCGGGCCTGCAAC |
| mCldn7-R | TCACACGTATTCCTTGGAGG | ||
| Claudin8 | 678 bp | mCldn8-F | CCATGGCAACCTACGCTCTTC |
| mCldn8-R | CTACACATACTGACTTTTGG | ||
| Claudin9 | 654 bp | mCldn9-F | CCATGGCTTCCACTGGCCTTG |
| mCldn9-R | TCACACATAGTCCCTCTTATC | ||
| Claudin10 | 689 bp | mCldn10a-F | CCATGTCCAGGGCACAGATCT |
| mCldn10b-F | CCATGGGTAGCACGGCCTTGG | ||
| 696 bp | mCldn10-R | TTAGACATAGGCATTTTTATC | |
| Claudin11 | 624 bp | mCldn11-F | CCATGGTAGCCACTTGCCTTC |
| mCldn11-R | TTAGACATGGGCACTCTTG | ||
| Claudin12 | 735 bp | mCldn12-F | CCATGGGCTGCCGAGATGTCC |
| mCldn12-R | TTAAGTGCTGTGTGAGACTAC | ||
| Claudin13 | 636 bp | mCldn13-F | CCATGGTCGTCAGCAAACAAG |
| mCldn13-R | TCAAACATCTAAGGTATCG | ||
| Claudin14 | 720 bp | mCldn14-F | CCATGGCCAGCACAGCGGTCC |
| mCldn14-R | TCACACGTAGTCATTCAGCC | ||
| Claudin15 | 684 bp | mCldn15-F | CATATGTCGGTAGCTGTGGAGACC |
| mCldn15-R | CTACACGTATGCGTTTTTGC | ||
| Claudin16 | 708 bp | mCldn16-F | CATATGAAGGATCTTCTTCAGTACG |
| mCldn16-R | TTATACTCTGGTGTCTACAGC | ||
| Claudin17 | 675 bp | mCldn17-F | CCATGGCTTTTTATCCCTTAC |
| mCldn17-R | TTAGACGTAGCTGGTGGAAG | ||
| Claudin18 | 150 bp | mCldn18-F | TTCATCATCTCCGGCATCTG |
| mCldn18-R | CAAAGGTGTACCTGGTCTGA | ||
| Claudin19 | 675 bp | mCldn19-F | CCATGGCCAACTCGGGCCTCCA |
| mCldn19-R | TTACACACCCAGGGGGCCCT | ||
| Claudin20 | 660 bp | mCldn20-F | ATGGCCTCGGCAGGTCTCC |
| mCldn20-R | TTACATGTAATCCTTCAAGTTATG | ||
| Claudin22 | 663 bp | mCldn22-F | CCATGGGCTTAGTCTTCCGAAC |
| mCldn22-R | TTAGATTTCTGGATTGGCT | ||
| Claudin23 | 891 bp | mCldn23-F | CATATGCGGACGCCGGTGGTGATGACG |
| mCldn23-R | CTACAGGTCGGAGTCACAGG | ||
| Claudin24 | 663 bp | mCldn24-F | CCATGGCTTTCATCTTCAGAACG |
| mCldn24-R | TTACACTTTAGGCTGTACAGTTCC | ||
| β-actin | 336 bp | mβ-actin-F | CACAGCTGAGAGGGAAATC |
| mβ-actin-R | TCAGCAATGCCTGGGTAC |