Figure 1.

Summary of the hPSC cardiac differentiation process.(A) Schematic approaches and growth factors/small molecules involved in hPSC cardiac differentiation. The five major cell types during hPSC cardiomyogenesis are shown: pluripotent stem cells, mesoderm progenitors, cardiac mesoderm cells, heart field progenitors, and cardiomyocytes. (B) Cardiac differentiation GiWi protocol is shown. hPSCs were first treated with a Gsk3 inhibitor to promote mesoderm differentiation and, then, on day 3 of differentiation, cells were treated with a Porcn inhibitor to block Wnt ligand function and, thus, inhibit Wnt signaling for enhancing cardiac differentiation.