Figure 5. Thylakoid fenestrations.
Alternate views of the 3D segmentations from (A–C) Figure 3 and (D) Figure 4 showing fenestrations in thylakoid stacks. In each example, there are holes at similar positions in multiple adjacent thylakoids, enabling free diffusion of stromal components from one side of the thylakoid stack to the other. In A and D, the fenestrations are not completely within the limited thickness of the FIB lamella, and hence appear like indentations in the top surfaces of the segmented thylakoid volumes.