Table 1.
Sequences of primers used for semi-quantitative real-time polymerase chain reaction
| Gene | Primer | Base sequence | PCR product (bp) |
| E2F-1 | Forward | 5'-CCCAACTCCCTCTACCCT-3' | 217 |
| Reverse | 5'-CTCCCATCTCATATCCATCCTG-3' | ||
| Survivin | Forward | 5'-AAATGCACTCCAGCCTCTGT-3' | 311 |
| Reverse | 5'-TGTCGAGGAAGCTTTCAGGT-3' | ||
| Bax | Forward | 5'-CCAAGAAGCTGAGCGAGTGT-3' | 269 |
| Reverse | 5'-CCGGAGGAAGTCCAATGTC-3' | ||
| Bcl-2 | Forward | 5'-GACTTCGCCGAGATGTCCAG-3' | 259 |
| Reverse | 5'-CATCCCAGCCTCCGTTATCC-3' | ||
| Cyclin D1 | Forward | 5'-CCCTCGGTGTCCTACTTCAA-3' | 237 |
| Reverse | 5'-GGGGATGGTCTCCTTCATCT-3' | ||
| Skp2 | Forward | 5'-GCTGCTAAAGGTCTCTGGTGT-3' | 291 |
| Reverse | 5'-AGGCTTAGATTCTGCAACTTG-3' | ||
| c-Myc | Forward | 5'-TTCTCTCCGTCCTCGGATTC-3' | 282 |
| Reverse | 5'-GTAGTTGTGCTGATGTGTGG-3' | ||
| GAPDH | Forward | 5'-ACCACAGTCCATGCCATCAC-3' | 450 |
| Reverse | 5'-TCACCACCCTGTTGCTGTA-3' |
GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; PCR: Polymerase chain reaction; Skp2: S-phase kinase-associated protein 2.