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. 2015 Jan 13;81(3):929–937. doi: 10.1128/AEM.03213-14

TABLE 1.

Microbial strains and plasmids used in this study

Strains or plasmid Description Antibiotic resistancea Reference or source
Strains
    AJ110637 Isolated wild-type strain (FERM BP-10955) 18
    ΔadhE mutant AJ110637 ΔadhE Km 18
    ES02 AJ110637 ΔadhE ΔldhA Km This work
    ES03 AJ110637 ΔadhE ΔldhA Δpta Km This work
    ES04 AJ110637 ΔadhE ΔldhA Δpta ΔbudA Km This work
Plasmids
    pKD46 λ Red-expressing plasmid Ap 30
    pRSFRedTER Broad-host-range λ Red-expressing plasmid Cm 31
    pMW-attLλ-Kmr-attRλ Cassette for gene disruption containing kanamycin resistance gene Km 31
    pMW-intxis-ts λ Int/Xis-expressing plasmid Ap 32, 33
    pRSF-Para-IX Broad-host-range λ Int/Xis-expressing plasmid Cm This work
    pSTV28 Plasmid vector with a replication origin of pACYC184 Cm TaKaRa BIO
    pSTV28-pyc Plasmid for expression of pyc from C. glutamicum Cm 18
    pSTV28-pck Plasmid for expression of pck from A. succinogenes Cm 18
    pSTV28-pck-pyc Plasmid for coexpression of A. succinogenes pck and C. glutamicum pyc Cm This work
a

Km, kanamycin; Ap, ampicillin; Cm, chloramphenicol.