TABLE 1.
Strains or plasmid | Description | Antibiotic resistancea | Reference or source |
---|---|---|---|
Strains | |||
AJ110637 | Isolated wild-type strain (FERM BP-10955) | 18 | |
ΔadhE mutant | AJ110637 ΔadhE | Km | 18 |
ES02 | AJ110637 ΔadhE ΔldhA | Km | This work |
ES03 | AJ110637 ΔadhE ΔldhA Δpta | Km | This work |
ES04 | AJ110637 ΔadhE ΔldhA Δpta ΔbudA | Km | This work |
Plasmids | |||
pKD46 | λ Red-expressing plasmid | Ap | 30 |
pRSFRedTER | Broad-host-range λ Red-expressing plasmid | Cm | 31 |
pMW-attLλ-Kmr-attRλ | Cassette for gene disruption containing kanamycin resistance gene | Km | 31 |
pMW-intxis-ts | λ Int/Xis-expressing plasmid | Ap | 32, 33 |
pRSF-Para-IX | Broad-host-range λ Int/Xis-expressing plasmid | Cm | This work |
pSTV28 | Plasmid vector with a replication origin of pACYC184 | Cm | TaKaRa BIO |
pSTV28-pyc | Plasmid for expression of pyc from C. glutamicum | Cm | 18 |
pSTV28-pck | Plasmid for expression of pck from A. succinogenes | Cm | 18 |
pSTV28-pck-pyc | Plasmid for coexpression of A. succinogenes pck and C. glutamicum pyc | Cm | This work |
Km, kanamycin; Ap, ampicillin; Cm, chloramphenicol.