TABLE 3.
Profiles of end pH, biomass, consumed glucose, and end products in multiple knockout mutants overexpressing PYC and/or PCKa
| Strainb | End pH | Biomass (g [DCW]/liter)c | Consumed glucose (g/liter) | End product (g/liter) |
Succinate yield (% [g/g])d | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Pyruvate | Malate | Formate | Succinate | Lactate | Acetate | Ethanol | 2,3-Butanediol | |||||
| ES02/empty | 5.8 ± 0.1 | 1.2 ± 0.1 | 3.4 ± 0.4 | 0.4 ± 0.1 | NDe | 0.7 ± 0.1 | 0.2 ± 0.1 | ND | 1.8 ± 0.1 | ND | 0.2 ± 0.1 | 5.9 ± 1.1 |
| ES02/PYC | 5.4 ± 0.1 | 1.6 ± 0.1 | 9.3 ± 0.2 | ND | ND | 0.2 ± 0.1 | 4.8 ± 0.1 | ND | 3.3 ± 0.1 | ND | 0.2 ± 0.1 | 51.6 ± 0.9 |
| ES02/PCK | 5.4 ± 0.1 | 1.5 ± 0.1 | 7.2 ± 0.4 | <0.1 | <0.1 | <0.1 | 4.6 ± 0.3 | <0.1 | 3.3 ± 0.1 | ND | 0.2 ± 0.1 | 63.9 ± 3.1 |
| ES03/empty | 5.7 ± 0.1 | 1.3 ± 0.1 | 3.7 ± 0.3 | 0.5 ± 0.1 | ND | 0.3 ± 0.1 | 0.3 ± 0.1 | ND | 0.5 ± 0.2 | <0.1 | 1.4 ± 0.2 | 9.1 ± 2.9 |
| ES03/PYC | 5.5 ± 0.1 | 1.5 ± 0.1 | 8.9 ± 0.5 | ND | ND | <0.1 | 3.8 ± 0.2 | <0.1 | 0.4 ± 0.1 | <0.1 | 3.1 ± 0.2 | 42.7 ± 2.5 |
| ES03/PCK | 5.4 ± 0.1 | 1.5 ± 0.2 | 7.4 ± 0.7 | 0.2 ± 0.1 | ND | 0.2 ± 0.1 | 4.2 ± 0.2 | ND | 0.5 ± 0.2 | <0.1 | 3.7 ± 0.2 | 56.8 ± 4.4 |
| ES04/empty | 5.7 ± 0.1 | 1.4 ± 0.1 | 3.1 ± 0.2 | 0.8 ± 0.1 | ND | 0.4 ± 0.1 | 0.5 ± 0.2 | ND | 0.8 ± 0.1 | <0.1 | <0.1 | 15.1 ± 2.8 |
| ES04/PYC | 5.5 ± 0.1 | 1.6 ± 0.1 | 6.2 ± 0.3 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 | 3.6 ± 0.3 | <0.1 | 0.4 ± 0.1 | <0.1 | <0.1 | 58.1 ± 4.2 |
| ES04/PCK | 5.5 ± 0.1 | 1.5 ± 0.1 | 5.8 ± 0.3 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 | 3.5 ± 0.3 | ND | 0.5 ± 0.1 | <0.1 | <0.1 | 60.3 ± 3.6 |
| ES02/PCK+PYC | 5.4 ± 0.1 | 1.5 ± 0.1 | 8.5 ± 0.4 | <0.1 | 0.2 ± 0.1 | <0.1 | 5.7 ± 0.3 | ND | 3.1 ± 0.1 | ND | 0.2 ± 0.1 | 67.0 ± 4.3 |
| ES03/PCK+PYC | 5.4 ± 0.1 | 1.5 ± 0.2 | 9.7 ± 0.9 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 | 5.9 ± 0.2 | ND | 0.7 ± 0.2 | <0.1 | 3.4 ± 0.2 | 60.8 ± 4.8 |
| ES04/PCK+PYC | 5.4 ± 0.1 | 1.6 ± 0.1 | 11.4 ± 0.9 | 0.3 ± 0.1 | 0.5 ± 0.1 | <0.1 | 8.1 ± 0.4 | <0.1 | 0.6 ± 0.1 | <0.1 | <0.1 | 71.0 ± 2.8 |
a
Data are expressed as means ± SD from four independent experiments.
b
All strains were cultivated for 48 h.
c
Initial biomass was adjusted to 1.2 g (DCW/liter).
d
Succinate yield is gram of product per gram of consumed glucose expressed as a percentage.
e
ND, not determined.