TABLE 3.
COMSTAT analysis of 4-day biofilms
| Parameter | Value for straina |
||||
|---|---|---|---|---|---|
| A34 | lpcA mutant | 3841 | lpcB mutant | lpsD mutant | |
| Average thickness (μm) | 28 ± 6 | 103 ± 12** | 31 ± 6 | 103 ± 16* | 110 ± 18* |
| Roughness coefficient | 0.41 ± 0.06 | 0.23 ± 0.02* | 0.66 ± 0.03 | 0.45 ± 0.06 | 0.31 ± 0.03* |
| Surface-to-vol ratio (μm2/μm3) | 0.12 ± 0.01 | 0.05 ± 0.00* | 0.08 ± 0.00 | 0.04 ± 0.00 | 0.04 ± 0.01 |
| % of the area covered by bacteria in each layer | |||||
| Layer 1 | 26.9 ± 3.2 | 7.8 ± 5.4* | 26.8 ± 5.7 | 7.5 ± 1.5* | 4.0 ± 2.0* |
| Layer 15 | 88.9 ± 0.2 | 48.7 ± 5.9* | 88.8 ± 7.9 | 39.6 ± 6.9* | 38.2 ± 2.6** |
| Layer 50 | 3.9 ± 0.7 | 23.8 ± 9.0*** | 5.9 ± 2.6 | 40.0 ± 3.8* | 12.7 ± 1.7 |
| Layer 150 | 3.0 ± 2.7 | 1.3 ± 0.7 | 3.0 ± 1.3 | ||
a
Values are means of data from at least 5 independent experiments. Parameters were calculated using COMSTAT and statistical analysis by GraphPad Prism 5 software (one-way analysis of variance). *, P < 0.05; **, P < 0.01; ***, P < 0.001.