TABLE 1.

Comparison of titers of Wolbachia bacteria found in F₁ chic²²¹/+ Drosophila female backgrounds and in comparable control (wild-type) flies^a

Drosophila background	Relative quantity of gene:		Pfaffl ratio compared to control	Relative ratio of expression
	wsp	rpl32
Control (stock 145)	13.98	15.57		4.37
F1 chic221/+	18.98	19.61	0.33	1.55

^aRelative quantity of the Wolbachia specific gene (wsp) compared to the host gene reference (rpl32) across five individual mutant flies and five individual control flies (stock 145, from which the infection within chic²²¹/+ was derived). The relative ratio of wsp expression is statistically significantly reduced in the heterozygous mutant background (t = 15.586, df = 6, P < 0.001).