Table 1.
Threshold values and parameters estimated for WT and PTM VDAC activity.
| WT (n = 4) | PPN 25 μMa (n = 5) | PPN 100 μMa (n = 5) | rVDAC (n = 3) | S137E (n = 3) | |
|---|---|---|---|---|---|
| THRESHOLD VALUES (pA) | |||||
| IC | −20 | −9 | −38 | −21.5 | −20 |
| IO | −15 | −6.7 | −34 | −14.5 | −15 |
| RATE CONSTANTS (s−1)b | |||||
| q13 | 141.6 ± 6.4 | 230 ± 1.8 | 120 ± 1.3 | 130.3 ± 14.8 | 135.3 ± 2.59 |
| q31 | 9.79 ± 4.8 | 3 ± 1.3 | 0.66 ± 0.27 | 47.5 ± 7.8 | 1.26 ± 0.49 |
| q15 | 177.3 ± 9.7 | 95 ± 1 | 139 ± 1.3 | 118.6 ± 14.8 | 152.2 ± 9.13 |
| q51 | 148.1 ± 5.5 | 220 ± 1.1 | 118 ± 1 | 161.2 ± 8 | 137.4 ± 20.17 |
| q34 | 253.3 ± 11.0 | 110 ± 1.1 | 157 ± 0.85 | 237.2 ± 8.1 | 169.9 ± 2.5 |
| q43 | 0.24 ± 0.12 | 0.12 ± 0.21 | 0.094 ± 0.02 | 0.36 ± 0.33 | 0.61 ± 0.2 |
| q24 | 332.6 ± 9.5 | 0.46 ± 0.13 | 165 ± 7 | 296.1 ± 13 | 143.8 ± 19.8 |
| q42 | 0.4 ± 0.07 | 0.048 ± 0.014 | 0.075 ± 0.016 | 3.6 ± 0.8 | 0.83 ± 0.29 |
a
These parameters are estimated by simultaneous fitting of all the dataset within each data-type.
b
These rate constant values are the mean of the representative parameters, such as shown in Figure 4B, obtained after fitting the Markov model against the WT and PTM VDAC data.