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. 2014 Dec 2;35(2):319–324. doi: 10.3892/ijmm.2014.2018

Figure 6.

Figure 6

Co-immunoprecipitation and western blot anlaysis were performed to detect interactions between X or X mutant proteins with cytochrome c oxidase subunit III (COXIII) in COS7 cells. Lane 1, western blot analysis using hemagglutinin (HA) antibody shows that a protein of approximately 17 kDa (the predicted size of HA-X) was co-immunoprecipitated with v-myc myelocytomatosis viral oncogene homologue (Myc) antibody from an extract of COS7 cells containing HA-X and Myc-COXIII fusion proteins; lane 2, western blot analysis of an extract from COS7 cells transformed with pCMV-HA-X 1 and pCMV-Myc-COXIII plasmids; lane 3, western blot analysis using HA antibody showing a protein of approximately 14 kDa (the predicted size of HA-X2) was co-immunoprecipitated with Myc antibody from an extract of COS7 cells containing HA-X2; lane 4, western blot analysis of an extract from COS7 cells transformed with plasmids pCMV-HA-X and pCMV-Myc; lane 5, western blot analysis of an extract from COS7 cells transformed with plasmids pCMV-HA and pCMV-Myc; lane 6, western blot analysis of an extract from non-transfected COS7 cells.