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. 2014 Dec 10;35(2):349–357. doi: 10.3892/ijmm.2014.2032

Figure 3.

Figure 3

TNF-α-induces NF-κB activation and IκB-α phosphorylation in human SH-SY5Y cells. SH-SY5Y cells were treated with 20 ng/ml TNF-α for 0, 6, 12 and 24 h. (A and B) Cells were lysed following TNF-α treatment, and cell extracts were prepared and subjected to immunostaining for the p65 subunit of NF-κB or β-actin. (C and D) Whole cell lysates of TNF-α treated cells were immunoblotted for phosphorylated-IκB-α, total IκB-α and β-actin. β-actin was used as the loading control. Quantification of the target protein bands relative to β-actin is shown in the right panel. (*P<0.05 vs. untreated group). TNF-α, tumor necrosis factor-α; NF-κB, nuclear factor-κB.