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. 2014 Dec 10;35(2):349–357. doi: 10.3892/ijmm.2014.2032

Figure 4.

Figure 4

TNF-α promotes CXCR4 expression through NF-κB pathway activition in SH-SY5Y cells. (A and B) Cells were treated with TNF-α (20 ng/ml) for various time, as indicated. (C and D) Cells were treated with TNF-α for various concentrations, as indicated, for 24 h. (E and F) Cells were treated with NF-κB inhibitor PDTC (25 and 50 μM) for 2 h and were subsequently treated with TNF-α (20 ng/ml) for 24 h. CXCR4 expression was analyzed by RT-qPCR (A, C and E) and western blot analysis (B, D and F). GAPDH and β-actin were used as the loading control. (*P<0.05 vs. untreated group, #P<0.05 vs. TNF-α-treated group). TNF-α, tumor necrosis factor-α; CXCR4, CXC chemokine receptor-4; NF-κB, nuclear factor-κB; PDTC, pyrrolidinedithiocarbamic acid ammonium salt; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.