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. 2014 Nov 24;35(2):311–318. doi: 10.3892/ijmm.2014.2008

Figure 1.

Figure 1

Overexpression of miR-141 inhibits EMT by downregulation of vimentin and FSP1 and upregulation of E-cadherin. (A) HK-2 cells were transfected with negative scrambled control miRNA (miR-SCR) or with miR-141, and were treated with or without TGF-β1 (2 ng/ml) for 48 h. The cell lysates were collected and immunoblotted with vimentin, FSP1 and E-cadherin antibodies as indicated. The figure is a representative of three experiments with similar results. (B–D) The western blotting results in A were quantified and shown in a graph format. The expression of the EMT markers (vimentin, FSP1 and E-cadherin) was normalized to β-actin, and the expression for the different groups was determined as a relative change from miR-SCR in the absence of TGF-β1 treatment and shown as mean ± standard error. EMT, epithelial-mesenchymal transition; FSP1, fibroblast-specific protein 1; TGF, transforming growth factor.