Figure 3.

Comparative qRT-PCR analyses of planktonic and surface attached cells. (A) Investigation of two representative housekeeping genes encoding RNA polymerase subunit B' (rpoB1) and the translation elongation factor 2 (aef2). Relative expression was normalized to external standard bgaH RNA. (B) Relative transcriptional quantification of the assembly ATPase encoding genes of the archaellum (flaI) and the type IV pilus biogenesis complexes pil-1 (pilB1) and pil-2 (pilB2) as well as the constitutively expressed ferredoxin gene (fdx). The bars represent the fold change of gene expression shown in base 2 logarithmic scale in adherent cells compared to the planktonic state, which is defined by the baseline.