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. 2015 Jan 13;5:755. doi: 10.3389/fmicb.2014.00755

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Copyright © 2015 Losensky, Vidakovic, Klingl, Pfeifer and Fröls.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypic characterization of the Hbt. salinarum R1, ΔflaI and ΔflaI/ΔpilB1 mutant strains. (A) Transmission electron micrographs of surface attached cells on carbon coated gold grids after 10 days of cultivation at 42°C. Pili-like surface structures observed with ΔflaI are labeled with arrows. (B) Frequency distribution of 100 filaments diameters in nm found with the ΔflaI mutant. (C) Light micrographs of Hbt. salinarum R1 and mutant strains attached to a glass surface after 10 days of cultivation at 42°C. Cells not attached to the surface were removed by stringent washing. Scale bars are 10 μm.