Table 1.
Primer sequences used in the qPCR experiments
| Gene name | Primer sequences (5’–3’) | Expected size(bp) | Accession Number (GenBank) |
|---|---|---|---|
| Kiss1 | Forward TGCTGCTTCTCCTCTGTGTGG | 110 | NM_181692.1 |
| Reverse ATTAACGAGTTCCTGGGGTCC | |||
| Kiss1r | Forward CTTTCCTTCTGTGCTGCGTA | 102 | NM_023992.1 |
| Reverse CCTGCTGGATGTAGTTGACG | |||
| GDF9 | Forward GAGGAAACACAGGTGGATTGAGATG | 174 | NM_021672.1 |
| Reverse AGATACAAGATGAGCGAGGGCG | |||
| BMP15 | Forward GCTAAAATGGTGAGGCTGATAAA | 180 | NM_021670.1 |
| Reverse ATGGCAGGAGAGATGGTAATG | |||
| FSHR | Forward CTACACATTGACAGCCATCACCCTA | 121 | NM_199237.1 |
| Reverse GGCAAAAGTCCAGCCCAATACC | |||
| PTGS2 | Forward TCTCCAACCTCTCCTACTACACCA | 139 | NM_017232.3 |
| Reverse ATGAACTCTCTCCTCAGAAGAACCT | |||
| HPRT1 | Forward CCCCAAAATGGTTAAGGTTGC | 175 | NM_012583.2 |
| Reverse TCCACTTTCGCTGATGACACAA |