Evaluation of the ability
of compounds to inhibit IL-1α,
LPS, or CL097-induced phosphorylation of p105, p38, and JNK in several
cell types. (a–c) 293 IL-1R, MEFs, and RAW cells were pretreated
with 1 at different concentrations for 1 h and then stimulated
with IL-1α or LPS for 30 min. Samples were collected and subjected
to Western blot for phosphorylation of p105, p38, JNK1/2, and total
p38. (d) Gen2.2 cells were pretreated with 1, S1, and 16 at 1 μM for 1 h and then stimulated with
CL097 for 30 min. Samples were collected and subjected to Western
blot.