FIG. 1.

Effect of Naproxen on type X collagen (COL X) expression in normal and osteoarthritis (OA) hMSCs. Normal hMSCs (Lonza) and the MSCs isolated from bone marrow aspirates of OA human donors undergoing total hip anthroplasty were cultured in DMEM (1% FBS and 1% PS) without or with Naproxen (100 μg/mL) for up to 72 h. COL X protein expression was determined by immunoblotting. GAPDH was used as a housekeeping gene and utilized to normalize the results. Values represent the mean±SE of four experiments (*p<0.05; ***p<0.001 compared with untreated normal hMSCs; ^###p<0.001 compared with untreated OA hMSCs). DMEM, Dulbecco's modified Eagle's medium; FBS, fetal bovine serum; hMSCs, human mesenchymal stem cells.