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. 2014 Oct 21;21(1-2):234–245. doi: 10.1089/ten.tea.2014.0148

FIG. 10.

FIG. 10.

Effect of 5-lipoxygenase inhibitor (MK-886) on Naproxen-induced RUNX2 gene expression in normal and OA hMSCs. Normal hMSCs (Lonza) and the hMSCs isolated from bone marrow aspirates of OA human donors undergoing total hip anthroplasty were cultured in complete DMEM to near confluence and were serum deprived for 24 h followed by treatment without or with 5-LOX inhibitor (MK-886, 10 μM) and Naproxen (100 μg/mL). RUNX2 gene expression was measured after 24 h of treatment by real-time RT-PCR. GAPDH was used as the housekeeping gene to normalize the results. Normal hMSC values represent the mean±SE of three experiments (*p<0.05, compared with Naproxen-treated normal hMSCs without 5-LOX inhibitor). OA hMSC values represent the mean±SE of six donors (###p<0.001, compared with Naproxen-treated OA hMSCs without 5-LOX inhibitor).