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. 2014 Oct 21;21(1-2):234–245. doi: 10.1089/ten.tea.2014.0148

FIG. 3.

FIG. 3.

Effect of Naproxen on phosphorylation of MAPKs in normal hMSCs. Normal hMSCs were cultured to near confluence in complete DMEM. Then, the cells were serum starved for 24 h followed by treatment with Naproxen (100 μg/mL) or without (CTL) for up to 6 h. Protein expression and phosphorylation of ERK 1/2, JNK, and p38 were determined by immunoblotting and protein bands were quantified using Image J (NIH) software. Total ERK 1/2, JNK, and p38 were used to normalize corresponding phosphorylated forms. Values represent the mean±SE of three experiments. (A) Effect of Naproxen on phosphorylation of extracellular signal-regulated kinase (ERK). Naproxen significantly increased the phosphorylation of ERK at 1 h (153±12, *p<0.05), which reached to below control levels after 6 h (50±26). (B) Effects of Naproxen on phosphorylation of c-Jun N-terminal kinase (JNK). Naproxen significantly increased the phosphorylation of JNK in 0.5 h (119±3, *p<0.05), which reached to control levels after 6 h. (C) Effects of Naproxen on phosphorylation of p38. The phosphorylation of p38 increased by 30 min and this increase was statistically significant at 6 h (282±106, **p<0.01).