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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Exp Neurol. 2014 Oct 29;263:325–338. doi: 10.1016/j.expneurol.2014.10.018

Figure 8. Regenerated peripheral nerves in MCT1 Het mice have delayed and incomplete re-myelination.

Figure 8

Uncrushed sural nerves from wild-type (WT; n=475 axons) and MCT1 Het (Het; n=964 axons) mice have identical G-ratios (A, C), while 6 week regenerated sural nerves from Het mice (n=770 axons) have significantly increased G-ratios compared to regenerated WT nerves (B, C; n=430 axons), reflecting thinner myelination. Electrophysiologically (D), the compound motor action potential (CMAP) latency from regenerating sciatic nerves from Het mice, but not uncrushed nerves, are prolonged compared to WT mice, reflecting reduced remyelination. Electron photomicrographs of sural nerves 1 weeks post-crush from WT (E) and Het (F) mice; 2 weeks post-crush from WT (G) and Het (H) mice; and 3 weeks post-crush from WT (I) and Het (J) mice, as well as from tibial nerves 3 weeks post-crush from WT (K) and Het (L) mice. An early myelin remnant is present in sural nerve from Het mice 1 week following sciatic nerve crush (F). Thinly myelinated or unmyelinated large axons (arrowheads) are seen in both sural (J) and tibial (L) nerves from MCT1 Het, but not WT (I, K), mice 3 weeks post-crush. Scale bars are all 2 μm. All error bars reflect S.E.M. * p<0.05, ns non-significant