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A single affinity state could not account for cyclase and internalization responses of δ opioid receptor agonists. (A) δ opioid receptor internalization and (B) cAMP accumulation data shown in Figure 5 were fit with the operational model, except that KA values for the agonists were fixed from published references as follows: KASNC-80: 5.75 nM; KAUFP-512: 100 pM; and KADPDPE: 12.02 nM. (Modified with permission from Charfi et al., 2013).
