Fig. 4.

RAB1A knock down results in reduced IEV presence in a cell. HeLa cells were transfected with siRNA targeting VP16 or RAB1A for 48 h then infected with VACV at MOI=5 for 8 h before being fixed with 4% PFA, 2.5% gluteraldehyde in 0.2M PIPES and processed for TEM. (A, C) Images of cytoplasmic viral factories containing multiple IMVs. (B) Image of the surface of a cell with the arrowhead pointing to a CEV on the cell surface, and arrows pointing to two CEVs on the tips of actin tails. (D–F) High magnification images of IEVs within VP16 siRNA transfected cells. (G) High magnification image of two IMVs within a RAB1A siRNA transfected cell for comparison. Scale bars for images A–C=214 nm, for images D–G=88 nm.