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. 1975 Sep;8(3):238–242. doi: 10.1128/aac.8.3.238

Biochemical Properties of a Penicillin Beta-Lactamase Mediated by R Factor from Bordetella bronchiseptica

Satoshi Yaginuma *, Nobuyuki Terakado †, Susumu Mitsuhashi †
PMCID: PMC429300  PMID: 241286

Abstract

The β-lactamase specified by an Rte16 plasmid in Bordetella bronchiseptica was purified 200-fold by carboxymethyl-Sephadex column chromatography and electrofocusing. The enzyme has a molecular weight of 46,000 ± 3,000 and an isoelectric point of 8.3 and was highly active against phenethicillin, oxacillin, and propicillin. The enzyme activity was inhibited by sodium chloride but not by ferrous ion. The maximal enzyme activity to benzylpenicillin was observed at pH 7.0 to 7.5 and at 40 C. It is concluded that this enzyme is different from the R-mediated β-lactamases, i.e., the type I and type II β-lactamases which have been classified in previous papers.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson E. S., Lewis M. J. Drug resistance and its transfer in Salmonella typhimurium. Nature. 1965 May 8;206(984):579–583. doi: 10.1038/206579a0. [DOI] [PubMed] [Google Scholar]
  2. Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964 May;91(2):222–233. doi: 10.1042/bj0910222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dale J. W. Characterization of the -lactamase specified by the resistance factor R-1818 in E. coli K12 and other Gram-negative bacteria. Biochem J. 1971 Jul;123(4):501–505. doi: 10.1042/bj1230501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dale J. W., Smith J. T. A direct comparison of two unusual R-factor-mediated -lactamases. Biochem J. 1972 Jun;128(1):173–174. doi: 10.1042/bj1280173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dale J. W., Smith J. T. The purification and properties of the -lactamase specified by the resistance factor R-1818 in Escherichia coli and Proteus mirabilis. Biochem J. 1971 Jul;123(4):493–500. doi: 10.1042/bj1230493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Datta N., Kontomichalou P. Penicillinase synthesis controlled by infectious R factors in Enterobacteriaceae. Nature. 1965 Oct 16;208(5007):239–241. doi: 10.1038/208239a0. [DOI] [PubMed] [Google Scholar]
  7. Datta N., Richmond M. H. The purification and properties of a penicillinase whose synthesis is mediated by an R-factor in Escherichia coli. Biochem J. 1966 Jan;98(1):204–209. doi: 10.1042/bj0980204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Lindqvist R. C., Nordström K. Resistance of Escherichia coli to penicillins. VII. Purification and characterization of a penicillinase mediated by the R factor R1. J Bacteriol. 1970 Jan;101(1):232–239. doi: 10.1128/jb.101.1.232-239.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. NOVICK R. P. Micro-iodometric assay for penicillinase. Biochem J. 1962 May;83:236–240. doi: 10.1042/bj0830236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. PERRET C. J. Iodometric assay of penicillinase. Nature. 1954 Nov 27;174(4439):1012–1013. doi: 10.1038/1741012a0. [DOI] [PubMed] [Google Scholar]
  12. Richmond M. H., Sykes R. B. The beta-lactamases of gram-negative bacteria and their possible physiological role. Adv Microb Physiol. 1973;9:31–88. doi: 10.1016/s0065-2911(08)60376-8. [DOI] [PubMed] [Google Scholar]
  13. Sawai T., Mitsuhashi S., Yamagishi S. Drug resistance of enteric bacteria. XIV. Comparison of beta-lactamases in gram-negative rod bacteria resistant to alpha-aminobenzylpenicillin. Jpn J Microbiol. 1968 Dec;12(4):423–434. doi: 10.1111/j.1348-0421.1968.tb00415.x. [DOI] [PubMed] [Google Scholar]
  14. Sawai T., Takahashi K., Yamagishi S., Mitsuhashi S. Variant of penicillinase mediated by an R factor in Escherichia coli. J Bacteriol. 1970 Nov;104(2):620–629. doi: 10.1128/jb.104.2.620-629.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Terakado N., Azechi H., Ninomiya K., Shimizu T. Demonstration of R factors in Bordetella bronchiseptica isolated from pigs. Antimicrob Agents Chemother. 1973 May;3(5):555–558. doi: 10.1128/aac.3.5.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yamagishi S., O'Hara K., Sawai T., Mitsuhashi S. The purification and properties of penicillin beta-lactamases mediated by transmissible R factors in Escherichia coli. J Biochem. 1969 Jul;66(1):11–20. doi: 10.1093/oxfordjournals.jbchem.a129111. [DOI] [PubMed] [Google Scholar]

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