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. Author manuscript; available in PMC: 2015 Jan 13.
Published in final edited form as: Biochim Biophys Acta. 2014 Oct 25;1853(1):244–253. doi: 10.1016/j.bbamcr.2014.10.017

Fig. 1.

Fig. 1

I3C inhibits tumorigenic properties of HCC cells in vitro. (A) Chemical structure of I3C. (B, C) I3C reduces HCC cell proliferation in culture. 1 × 104 SK-Hep-1 cells and SNU-449 cells were seeded in 96-well plates, respectively, and incubated overnight to allow attachment before starting the treatments with DMSO alone or different doses of indole-3-carbinol (I3C) dissolved in DMSO. Cell viability was assessed at indicated time points by MTT. (D) Clonogenic survival of HCC cells decreased when exposed to I3C. 5 × 102 SK-Hep-1 and SNU-449 cells were separately seeded in 6-well plates and allowed to grow for 24 h. The cells were then treated with medium containing 50 and 100 μM of I3C or DMSO. After 2 weeks, HCC cells were washed with PBS twice, fixed with methanol, and then stained with crystal violet and counted. The data are mean ± SD values of triplicates from a three representative experiments (D, E) or mean ± SD values from three independent experiments (A, B), respectively (*P < 0.05; **P < 0.01).