. Author manuscript; available in PMC: 2015 Jan 13.

Published in final edited form as: J Biol Chem. 2006 Apr 6;281(23):16108–16116. doi: 10.1074/jbc.M600760200

TABLE 1.

Peptidyl substrates

	Peptide sequence	pH	K_m	k _cat	k_cat/K_m	k_cat/K_m(relative)
			μ m	s^–1	s^–1m^–1
PS-1^a	Abz-Ala-Lys-Arg-Arg-Thr-Lys-Arg¹⁰⁷ ↓ Asp-Val-Tyr(NO₂)-Ala	7.5	1.99	1.90	9.58 × 10⁵	1.000
		6.0	1.62	1.03	6.34 × 10⁵	0.661
PS-2^a	Abz-His-Arg-Gly-Val-Thr-Lys-Arg⁷⁵ ↓ Ser-Leu-Tyr(NO₂)-Ala	7.5	23.75	6.37	2.68 × 10⁵	0.279
		6.0	6.59	2.92	4.43 × 10⁵	0.458
PS-2 H69K	Abz-Lys-Arg-Gly-Val-Thr-Lys-Arg⁷⁵ ↓ Ser-Leu-Tyr(NO₂)-Ala	7.5	8.01	2.05	2.56 × 10⁵	0.267
		6.0	11.95	1.61	1.35 × 10⁵	0.141
PS-2 H69L	Abz-Leu-Arg-Gly-Val-Thr-Lys-Arg⁷⁵ ↓ Ser-Leu-Tyr(NO₂)-Ala	7.5	38.20	2.90	7.59 × 10⁴	0.079
		6.0	45.93	2.66	5.79 × 10⁴	0.060

Data are from Ref. 14.