Table 1.
Outline of experimental groups and procedures for the effect of varenicline on the expression of CPP study
| Experimental Groups |
All mice received two injections spaced 15 min apart on all days | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Day 1 |
Day 2 |
Day 3 |
Day 4 |
Day 5 |
Day 6 |
Day 7 |
Day 8 |
Day 9 |
Day 10 |
|
| Effect of Varenicline on the Expression of CPP | ||||||||||
| G+: 4 groups (VAR 0, 0.5, 1, and 1.5 mg/kg) | SAL, SAL (Black) | SAL, SAL (Hole) | SAL, E2 (Grid) | SAL, SAL (Hole) | SAL, E2 (Grid) | SAL, SAL (Hole) | SAL, E2 (Grid) | SAL, SAL (Hole) | SAL, E2 (Grid) | VAR, SAL (Grid+Hole) |
| G-: 4 groups (VAR 0, 0.5, 1, and 1.5 mg/kg) | SAL, SAL (Black) | SAL, SAL (Grid) | SAL, E2 (Hole) | SAL, SAL (Grid) | SAL, E2 (Hole) | SAL, SAL (Grid) | SAL, E2 (Hole) | SAL, SAL (Grid) | SAL, E2 (Hole) | VAR, SAL (Grid+Hole) |
Under Experimental Groups, the entry following G+: or G-: indicates the pretreatment received on the place preference test day (day 10) by 4 groups of mice in each case. Entries under Day 1 ā Day 9 indicate the two injections received and the type of flooring in the chamber during the 5-min trial (habituation on Day 1; acquisition conditioning on Day 2 ā Day 9). Entries under Day 10 indicate the two injections received and the floor types on which mice could choose to spend time. The effect of varenicline pretreatment on the expression of a place preference was assessed during a 30-min test on this day. Note that this table does not reflect all treatment conditions as the groups were also counterbalanced for side on which the grid floor was placed during the preference test, and whether ethanol or saline was the first conditioning session. CPP = conditioned place preference; G+ = mice conditioned with ethanol on the grid floor; Gā = mice conditioned with ethanol on the hole floor; E2 = 2 g/kg ethanol; SAL = 0.9% saline; VAR = varenicline.