Figure 2. NMR analysis of the EI-PEP and EI-αKG complexes.

Weighted combined chemical shift perturbations (Δ_H/N,C) induced by (a) PEP and (b) αKG on the ¹H-¹⁵N and ¹H-¹³C_methyl TROSY correlation spectra of EI^A displayed on the structure of EI as spheres with the relationship between size and color of each sphere and chemical shift perturbation depicted by the color bar. Δ_H/N,C values are displayed on one subunit of the EI dimer. The second subunit is shown as a transparent surface. The EIN and EIC domains are colored light blue and pink, respectively. PEP and αKG bound to one subunit of EI are shown as green sticks. Plots of of chemical shift perturbations as a function of residue for EI^WT, EI^Q and EI^A are shown in Supplementary Fig. 1.