FIG. 4.

Effect of diclofenac-sodium-releasing PLGA/PEG scaffolds on cell viability in the in vitro calvarial osteoblast inflammation model. Live/Dead™ viability assay of osteoblast monolayers after 7 days of culture with and without IL-1β, TNF-α, and IFN-γ, with PLGA/PEG scaffolds initially loaded with various masses of diclofenac sodium. (A) Representative images of osteoblast monolayers at day 7: live cells in green and dead cells in red. (B) Image quantification performed to determine average number of live and dead cells per image. Statistical significance of total cell number versus total cell number in 0 μg/scaffold in osteogenic medium, *p≤0.01. (C) Percentage of live cells in each group. Statistical significance versus 0 μg/scaffold in osteogenic medium, **p≤0.001, ***p≤0.0001. Statistical significance versus 0 μg/scaffold in cytokine medium, ^###p≤0.0001. Experiment repeated in triplicate, each with n=3. Color images available online at www.liebertpub.com/tea