FIG. 6.

Effect of diclofenac-sodium-releasing PLGA/PEG scaffolds on PGE₂ production in the in vitro calvarial osteoblast inflammation model. PGE₂ accumulation was measured on days 1, 2, and 7, after scaffold placed in transwell. (A) PGE₂ concentration at each timepoint. (B) Cumulative PGE₂ concentration over 7 days. Inset shows cumulative PGE₂ concentration excluding “no scaffold” groups. Statistical significance versus 0 μg/scaffold in osteogenic medium, *p≤0.01. Statistical significance versus 0 μg/scaffold in cytokine medium, ^#p≤0.01. Values represented as mean±SEM; experiment repeated in triplicate each with n=3.