FIG. 6.

Immunohistochemistry staining of neoskin after grafting on human skin equivalents. (A, B) Mouse skin with the printable skin graft at week 2. (C, D) Mouse skin with Apligraf skin graft at week 4. The tissues were stained with Anti-Nuclei Antibody, Clone 235-1, and visualized with Cy 3 conjugated AffiniPure Donkey Anti-Mouse IgG (H+L) (red). (A, C) DAPI staining for the nuclei of the cells in the tissue section (mouse and human cells), the white arrow heads show the presence of the microvessels in the mouse tissue. (B, D) The stained nuclei of the human cells present in the mouse skin (bright red). Color images available online at www.liebertpub.com/tea