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. 2014 Dec 30;7:39. doi: 10.1186/1756-8935-7-39

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© Stixová et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fluorescence intensity of GFP-UBF1 after UVA irradiation in non-treated and actinomycin D-treated cells. (A) Recruitment of GFP-UBF1 to UVA-induced DNA lesions in regions of interest (yellow) of (a) non-treated cells and (b) actinomycin D-treated cells. Images show normalized intensity of GFP-UBF1 fluorescence in UVA-induced DNA lesions in iMEFs treated with actinomycin D and non-treated iMEFs. Fluorescence intensity was measured for GFP-UBF1 in (B) irradiated nucleoplasm and (C) irradiated nucleoli. Approximately 40 nuclei were analyzed for each control and actinomycin D-treated cells. LCI, live-cell image.