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. 2014 Dec 30;7:39. doi: 10.1186/1756-8935-7-39

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© Stixová et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Protein interactions at DNA lesions. (A) FRET results for reference interacting partners p53 (green) and 53BP1 (red). (B) FRET analysis of UBF1/2 dimerization. FRET analysis of (C) UBF1/2 (red) and γH2AX (green), (D) GFP-UBF1 (green) and 53BP1 (red), and (E) HP1β (green) and UBF1/2 (red). Studies were performed in control and γ-irradiated cells using a Leica TSC SP5 confocal microscope. For each event, 10 nuclei were analyzed in three independent experiments. IF, immunofluorescence; non-irrad., non-irradiated cells.