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. 2014 Dec 30;7:39. doi: 10.1186/1756-8935-7-39

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© Stixová et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Functional analysis of HP1βΔCSD, HP1βΔhinge, and HP1βΔCD for UBF1 recruitment to UVA-induced DNA lesions. (A) Analysis of endogenous UBF1/2 (red) recruitment to UVA-induced DNA lesions (yellow regions of interest) in MEFs transiently expressing (a) GFP-HP1β-ΔCSD, (b) GFP-HP1β-Δhinge, or (c) GFP-HP1β-ΔCD (all green). (B) Quantification of fluorescence intensity of UBF1/2 (red) and HP1β (green) from panel A. Quantification was performed for (a) GFP-HP1β-ΔCSD, (b) GFP-HP1β-Δhinge, and (c) GFP-HP1β-ΔCD (all green). (C) Percentage of cells with recruitment of studied proteins to DNA lesions. Thirty cells were analyzed for each event in three independent experiments. (D) Analysis of endogenous HP1β (red) at UVA-induced DNA lesions (yellow regions of interest ) in mouse embryonic fibroblasts transiently expressing GFP-HP1β-ΔCSD (green). For panel D, 20 nuclei were analyzed. CD, chromodomain; CSD, chromo shadow domain; IF, immunofluorescence; LCI, live-cell image.